The aim of this study was to measure and identify the reactive carbonyl species (RCSs) released in the blood of humans subjected to hepatic resection. Pre-anesthesia malondialdehyde (MDA) plasma content (0.36 ± 0.11 nmol/mg protein) remained almost unchanged immediately after anaesthesia, before clamping and at the 10th min after ischemia, while markedly increased (to 0.59 ± 0.07 nmol/mg; p < 0.01, Tukey’s post test) at the 10th min of reperfusion. A similar trend was observed for the protein carbonyls (PCs), whose pre-anesthesia levels (0.17 ± 0.13 nmol/mg) did not significantly change during ischemia, while increased more than fourfold at the 10th min of reperfusion (0.75 ± 0.17 nmol/mg; p < 0.01, Tukey’s post test). RCSs were then identified as covalent adducts to the albumin Cys34, which we previously found as the most reactive protein nucleophilic site in plasma. By using a mass spectrometry (MS) approach based on precursor ion scanning, we found that acrolein (ACR) is the main RCS adducted to albumin Cys34. In basal conditions, the adducted albumin was 0.6 ± 0.4% of the native form but it increased by almost fourfold at the 10th min of reperfusion (2.3 ± 0.7%; p < 0.01, t-test analysis). Since RCSs are damaging molecules, we propose that RCSs, and ACR in particular, are new targets for novel molecular treatments aimed at reducing the ischemia/reperfusion damage by the use of RCS sequestering agents.
Albumin Cys34 adducted by acrolein as a marker of oxidative stress in ischemia-reperfusion injury during hepatectomy / E. Witort, S. Capaccioli, M. Becatti, C. Fiorillo, G. Batignani, V. Pavoni, M. Piccini, M. Orioli, M. Carini, G. Aldini, M. Lulli. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - 50:8(2016 Aug 02), pp. 831-839.
|Titolo:||Albumin Cys34 adducted by acrolein as a marker of oxidative stress in ischemia-reperfusion injury during hepatectomy|
ALDINI, GIANCARLO (Penultimo)
|Parole Chiave:||hepatic resection; mass spectrometry; protein carbonyls; reactive carbonyl species; biochemistry|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
Settore MED/04 - Patologia Generale
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore MED/18 - Chirurgia Generale
|Data di pubblicazione:||2-ago-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1080/10715762.2016.1179736|
|Appare nelle tipologie:||01 - Articolo su periodico|
File in questo prodotto:
|Albumin Cys34 adducted by acrolein as a marker of oxidative stress in ischemia reperfusion injury during hepatectomy.pdf||Publisher's version/PDF||Administrator Richiedi una copia|