We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in ones laboratory.
IPG with electrodic plateaus (and other unusual procedures for 2-DE) / E. Gianazza, A. Guerini Rocco, A. Marchetto, L. Vergani. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 28:16(2007), pp. 2953-2956.
IPG with electrodic plateaus (and other unusual procedures for 2-DE)
E. GianazzaPrimo
;A. Guerini RoccoSecondo
;
2007
Abstract
We describe some simple changes to the geometry of the IPG strips that make them suitable to the loading of very large sample volumes and of high-salt solutions. Of special relevance is the possibility of using strips with immobilized plateau(s) to either side of the gradient, or to both, also in connection with in-gel rehydration protocols and focusing in stock trays. The only requirement to achieve this is to leave the all-ready-made attitude and go back to custom polymerization of the IPGs in ones laboratory.Pubblicazioni consigliate
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