Intestinal uptake of abnormal prion protein (PrPSc), the pathological agent involved in transmissible spongiform encephalopathies (TSEs), has been investigated in rainbow trout (Oncorhynchus mykiss). Experimental procedures were conducted in vivo by immunohistological PrPSc localization in intestine and pyloric caeca after forced feeding of infected material. Results indicate that PrPSc was absorbed by the intestinal mucosa and that it persisted in the fish gastrointestinal tract for up to 3 days in pyloric caeca and for up to 7 days in the distal intestine. It did not remain longer than 15 days in the fish intestine; furthermore, it did not cross the intestinal barrier.

In vivo prion protein intestinal uptake in fish / A. Dalla Valle Zenone, M. Iriti, F. Faoro, C. Berti, S. Ciappellano. - In: APMIS. ACTA PATHOLOGICA, MICROBIOLOGICA ET IMMUNOLOGICA SCANDINAVICA. - ISSN 0903-4641. - 116:3(2008 Mar), pp. 173-180.

In vivo prion protein intestinal uptake in fish

A. Dalla Valle Zenone
Primo
;
M. Iriti
Secondo
;
F. Faoro;C. Berti
Penultimo
;
S. Ciappellano
Ultimo
2008-03

Abstract

Intestinal uptake of abnormal prion protein (PrPSc), the pathological agent involved in transmissible spongiform encephalopathies (TSEs), has been investigated in rainbow trout (Oncorhynchus mykiss). Experimental procedures were conducted in vivo by immunohistological PrPSc localization in intestine and pyloric caeca after forced feeding of infected material. Results indicate that PrPSc was absorbed by the intestinal mucosa and that it persisted in the fish gastrointestinal tract for up to 3 days in pyloric caeca and for up to 7 days in the distal intestine. It did not remain longer than 15 days in the fish intestine; furthermore, it did not cross the intestinal barrier.
Animal nutrition; Immunostaining; Intestinal uptake; Prion protein; Rainbow trout
Settore BIO/09 - Fisiologia
Settore AGR/12 - Patologia Vegetale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/38415
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