In an attempt to dissect endocytosis in Nicotiana tabacum L. pollen tubes, two different probes – positively or negatively charged nanogold – were employed. The destiny of internalized plasma membrane domains, carrying negatively or positively charged residues, was followed at the ultrastructural level and revealed distinct endocytic pathways. Time-course experiments and electron microscopy showed internalization of subapical plasmamembrane domains that were mainly recycled to the secretory pathway through the Golgi apparatus and a second mainly degradative pathway involving plasma membrane retrieval at the tip. In vivo time-lapse experiments using FM4-64 combined with quantitative analysis confirmed the existence of distinct internalization regions. Ikarugamycin, an inhibitor of clathrin-dependent endocytosis, allowed us to further dissect the endocytic process: electron microscopy and time-lapse studies suggested that clathrin-dependent endocytosis occurs in the tip and subapical regions, because recycling of positively charged nanogold to the Golgi bodies and the consignment of negatively charged nanogold to vacuoles were affected. However, intact positively charged-nanogold transport to vacuoles supports the idea that an endocytic pathway that does not require clathrin is also present in pollen tubes.
Distinct endocytic pathways identified in tobacco pollen tubes using charged nanogold / A. Moscatelli, F. Ciampolini, S. Rodighiero, E. Onelli, M. Cresti, N. Santo, A. Idilli. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 120:21(2007), pp. 3804-3819.
|Titolo:||Distinct endocytic pathways identified in tobacco pollen tubes using charged nanogold|
MOSCATELLI, ALESSANDRA (Primo)
SANTO, NADIA (Penultimo)
IDILLI, AURORA IRENE (Ultimo)
|Parole Chiave:||Endocytosis; Nicotiana tabacum (L.); Pollen tube|
|Settore Scientifico Disciplinare:||Settore BIO/02 - Botanica Sistematica|
Settore BIO/01 - Botanica Generale
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1242/jcs.012138|
|Appare nelle tipologie:||01 - Articolo su periodico|