Background: Age and short leukocyte telomeres have been associated with a higher risk of Alzheimer's disease (AD). Inflammation is involved in AD and it is suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these processes. Objective: The aim is to correlate telomere length (TL) in peripheral blood mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β (Aβ)-stimulated PBMC. Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly (HE). After a two-year follow-up period, patients were retrospectively evaluated as slow-progressing (ADS) (Mini Mental State Examination (MMSE) decline over the two years of follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was measured by flow cytometry and in vitro IL-10 production by enzyme-linked immunosorbent assay. Results: TL (mean±SD) for HE, ADS, and ADFwas 2.3±0.1, 2.0±0.1, and 2.5±0.1 Kb, respectively. ADS showed a shorter TL compared to HE (p = 0.034) and to ADF (p = 0.005). MMSE decline correlated with TL in AD (R2 = 0.284; p = 0.008). We found a significant difference in IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus 59.0±27.0; p = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE showed a trend toward significance (47.1±25.4 versus 55.3±27.9; p = 0.10). Conclusion: PBMC from ADF may be characterized by an impaired response induced by Aβ and by a reduced proliferative response responsible for the longer telomeres. TL might be a contributing factor in predicting the rate of AD progression.
Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression / E. Tedone, B. Arosio, F. Colombo, E. Ferri, D. Asselineau, F. Piette, C. Gussago, J. Belmin, S. Pariel, K. Benlhassan, M. Casati, A. Bornand, P.D. Rossi, P. Mazzola, G. Annoni, M. Doulazmi, J. Mariani, L. Porretti, D.H. Bray, D. Mari. - In: JOURNAL OF ALZHEIMER'S DISEASE. - ISSN 1387-2877. - 46:3(2015), pp. 761-769.
|Titolo:||Leukocyte telomere length in Alzheimer's disease patients with a different rate of progression|
TEDONE, ENZO (Corresponding)
AROSIO, BEATRICE (Secondo)
MARI, DANIELA (Ultimo)
|Parole Chiave:||Alzheimer's disease; disease progression; interleukin-10; peripheral blood mononuclear cells; telomere; Psychiatry and Mental Health; Geriatrics and Gerontology; Clinical Psychology|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3233/JAD-142808|
|Appare nelle tipologie:||01 - Articolo su periodico|
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