Ki67 can discriminate between high- and low-grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67-positive cells), evaluated by flow cytometry, in 40 dogs with high-grade B-cell lymphoma, treated with a modified Wisconsin-Madison protocol (UW-25). The following variables were investigated for association with lymphoma specific survival (LSS) and relapse free interval (RFI): Ki67%, breed, sex, age, stage, substage, complete remission (CR). By multivariate analysis, Ki67% (P=0.009) and achievement of CR (P=0.001) were independent prognostic factors for LSS. Dogs with intermediate Ki67% (20.1-40%) presented longer LSS and RFI (median=866 and 428 days, respectively) than dogs with low (median=42days, P<0.001; median=159days, P=0.014) or high (median=173days, P=0.038; median=100days, P=0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high-grade B-cell lymphoma.
Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma / A. Poggi, B. Miniscalco, E. Morello, F. Gattino, A. Delaude, L. Ferrero Poschetto, L. Aresu, M.E. Gelain, V. Martini, S. Comazzi, F. Riondato. - In: VETERINARY AND COMPARATIVE ONCOLOGY. - ISSN 1476-5810. - (2016). [Epub ahead of print] [10.1111/vco.12184]
Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma
V. Martini;S. ComazziPenultimo
;
2016
Abstract
Ki67 can discriminate between high- and low-grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67-positive cells), evaluated by flow cytometry, in 40 dogs with high-grade B-cell lymphoma, treated with a modified Wisconsin-Madison protocol (UW-25). The following variables were investigated for association with lymphoma specific survival (LSS) and relapse free interval (RFI): Ki67%, breed, sex, age, stage, substage, complete remission (CR). By multivariate analysis, Ki67% (P=0.009) and achievement of CR (P=0.001) were independent prognostic factors for LSS. Dogs with intermediate Ki67% (20.1-40%) presented longer LSS and RFI (median=866 and 428 days, respectively) than dogs with low (median=42days, P<0.001; median=159days, P=0.014) or high (median=173days, P=0.038; median=100days, P=0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high-grade B-cell lymphoma.
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