Background: Prox1, the vertebrate homolog of prospero in Drosophila melanogaster, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. Results: Here we report that, in zebrafish, prox1 is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced prox1 expression in a group of neurons, already positive for otp1, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic prox1/otp1 overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium. Conclusion: Our findings indicate that prox1 activity is crucial for the proper development of the otp1-positive hypothalamic neuronal precursors to their terminal CA phenotype.

Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development / A. Pistocchi, G. Gaudenzi, S. Carra, E. Bresciani, L. Del Giacco, F. Cotelli. - In: BMC DEVELOPMENTAL BIOLOGY. - ISSN 1471-213X. - 8:27(2008). [10.1186/1471-213X-8-27]

Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development

A. Pistocchi
Primo
;
G. Gaudenzi
Secondo
;
S. Carra;E. Bresciani;L. Del Giacco
Penultimo
;
F. Cotelli
Ultimo
2008

Abstract

Background: Prox1, the vertebrate homolog of prospero in Drosophila melanogaster, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. Results: Here we report that, in zebrafish, prox1 is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced prox1 expression in a group of neurons, already positive for otp1, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic prox1/otp1 overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium. Conclusion: Our findings indicate that prox1 activity is crucial for the proper development of the otp1-positive hypothalamic neuronal precursors to their terminal CA phenotype.
Settore BIO/06 - Anatomia Comparata e Citologia
2008
http://www.biomedcentral.com/1471-213X/8/27
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/36515
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