T acute lymphoblastic leukemia cell lines treated with hexamethylene bisacetamide (HMBA) undergo a delay in cell cycle progression and increase susceptibility to apoptosis, although they never overcome the differentiation block. In accordance with changes in cell cycle and apoptosis, transitory p53 pathway activation commonly occurs. Bcl-2 inhibition further favours the pro-apoptotic effect of HMBA. Notch1 expression is down regulated by reduction of its transcription level. Accordingly, Notch1 protein and transcriptional activity were affected. Even if HMBA generally reduces Notch1 level in T acute lymphoblastic leukemia (T-ALL) cell lines, this does not commonly influence the biological response; in fact all the analysed cell lines, except CEM cells, display no biological effect following DAPT-induced Notch inhibition.

Hexamethylene bisacetamide inhibits malignant phenotype in T-ALL cell lines / V. Cecchinato, E. Erba, A. Basile, B. Scarpati, C. Fazi, B. Brando, P. Comi, R. Chiaramonte. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 32:5(2008), pp. 791-797. [10.1016/j.leukres.2007.09.008]

Hexamethylene bisacetamide inhibits malignant phenotype in T-ALL cell lines

V. Cecchinato
Primo
;
A. Basile;P. Comi
Penultimo
;
R. Chiaramonte
Ultimo
2008

Abstract

T acute lymphoblastic leukemia cell lines treated with hexamethylene bisacetamide (HMBA) undergo a delay in cell cycle progression and increase susceptibility to apoptosis, although they never overcome the differentiation block. In accordance with changes in cell cycle and apoptosis, transitory p53 pathway activation commonly occurs. Bcl-2 inhibition further favours the pro-apoptotic effect of HMBA. Notch1 expression is down regulated by reduction of its transcription level. Accordingly, Notch1 protein and transcriptional activity were affected. Even if HMBA generally reduces Notch1 level in T acute lymphoblastic leukemia (T-ALL) cell lines, this does not commonly influence the biological response; in fact all the analysed cell lines, except CEM cells, display no biological effect following DAPT-induced Notch inhibition.
Apoptosis; Bax; Bcl-2; Cell cycle; HMBA; Notch1; p53; T-ALL
Settore MED/04 - Patologia Generale
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/35662
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