Calendula officinalis L. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2 extract obtained from C. officinalis flowers was evaluated in vitro on the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2 extract. The main components of fraction J were loliolide and the fucoside acetates of β-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-κB driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences that Calendula officinalis L. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-κB system, identifying the compounds responsible, at least in part, for the observed effect.
A bio-guided fractionation to assess the inhibitory activity of Calendula officinalis L. on the NF-κB driven transcription in human gastric epithelial cells / E. Colombo, E. Sangiovanni, M. D'Ambrosio, E. Bosisio, A. Ciocarlan, M. Fumagalli, A. Guerriero, P. Harghel, M. Dell'Agli. - In: EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE. - ISSN 1741-4288. - 2015(2015 Sep), pp. 727342.1-727342.8.
|Titolo:||A bio-guided fractionation to assess the inhibitory activity of Calendula officinalis L. on the NF-κB driven transcription in human gastric epithelial cells|
|Parole Chiave:||NF-kB; inflammation; Calendula officinalis; marigold; gastric epithelial cells|
|Settore Scientifico Disciplinare:||Settore BIO/15 - Biologia Farmaceutica|
Settore BIO/14 - Farmacologia
Settore CHIM/06 - Chimica Organica
|Data di pubblicazione:||set-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1155/2015/727342|
|Appare nelle tipologie:||01 - Articolo su periodico|