Oxidative polymerization of tyrosol by horseradish peroxidase (HRP)-H2O2 afforded an insoluble product (oligotyrosol, OligoTyr) consisting of mixture of linear oligomers (up to 11-mer) with limited benzylic branching points, as evidenced by ESI-MS and solid state C-13 NMR analysis. OligoTyr proved to be significantly more active than tyrosol in several antioxidant assays and was not toxic to human osteosarcoma SaOS-2 cells, stimulating alkaline phosphatase (ALP) activity at day 7 in a similar manner as tyrosol. However, when loaded at 5% w/w into highly porous polylactic acid (PLA) scaffolds featuring hierarchical structures, OligoTyr caused a significant increase in the ALP activity of SaOS-2 cells compared to PLA alone, while tyrosol was completely inactive. A release of ca. 5% from PLA was determined after 1 week in a physiological medium. No significant influence on calcium release from PLA scaffolds containing 5% beta-tricalcium phosphate was observed.
Powering tyrosol antioxidant capacity and osteogenic activity by biocatalytic polymerization / S. Antenucci, L. Panzella, H. Farina, M.A. Ortenzi, E. Caneva, S. Martinotti, E. Ranzato, B. Burlando, M. D'Ischia, A. Napolitano, L. Verotta. - In: RSC ADVANCES. - ISSN 2046-2069. - 6:4(2016), pp. 2993-3002.
|Titolo:||Powering tyrosol antioxidant capacity and osteogenic activity by biocatalytic polymerization|
ANTENUCCI, STEFANO (Primo)
VEROTTA, LUISELLA (Ultimo)
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
Settore CHIM/04 - Chimica Industriale
|Data di pubblicazione:||2016|
|Data ahead of print / Data di stampa:||22-dic-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1039/C5RA23004G|
|Appare nelle tipologie:||01 - Articolo su periodico|