Ile-Ala-Val-Pro-Gly-Glu-Val-Ala (IAVPGEVA), Ile-Ala-Val-Pro-Thr-Gly-Val-Ala (IAVPTGVA) and Leu-Pro-Tyr-Pro (LPYP), three peptides deriving from soy glycinin hydrolysis, are known to regulate cholesterol metabolism in human hepatic HepG2 cells. We have recently demonstrated that the mechanism of action involves the activation of adenosine monophosphate-activated protein kinase (AMPK). This fact suggested a potential activity of the same peptides on glucose metabolism that prompted us to also investigate this aspect in the same cells. After treatment with IAVPGEVA, IAVPTGVA and LPYP, HepG2 cells were analyzed using a combination of molecular techniques, including western blot analysis, glucose uptake experiments and fluorescence microscopy evaluation. The results showed that these peptides are indeed able to enhance the capacity of HepG2 cells to uptake glucose, via glucose transporter 1 GLUT1 and glucose transporter 4 GLUT4 activation, through the stimulation of protein kinase B Akt and adenosine monophosphate-activated protein kinase AMPK pathways, both involved in glucose metabolism.
Three Peptides from Soy Glycinin Modulate Glucose Metabolism in Human Hepatic HepG2 Cells / C. Lammi, C. Zanoni, A. Arnoldi. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 16:11(2015), pp. 27362-27370.
|Titolo:||Three Peptides from Soy Glycinin Modulate Glucose Metabolism in Human Hepatic HepG2 Cells|
LAMMI, CARMEN (Primo)
ZANONI, CHIARA (Secondo)
ARNOLDI, ANNA (Ultimo)
|Parole Chiave:||HepG2 cell line; bioactive peptides; functional foods; glucose uptake; plant proteins|
|Settore Scientifico Disciplinare:||Settore CHIM/10 - Chimica degli Alimenti|
|Progetto:||Innovative functional foods based on sweet lupin protein for cardiovascular prevention|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ijms161126029|
|Appare nelle tipologie:||01 - Articolo su periodico|