The measurement of the intensity of light reflected by interfaces with extremely low reflectivity in water enables the label-free, multiplex quantification of the binding between immobilized probes (e.g. antibodies) and targets in solution using extremely simple instrumental set-up. Here we show that, despite its simplicity, the method enables label-free detection in complex samples characterized by high absorbance and turbidity. Diagnostic markers of Tomato spotted wilt virus are revealed in crude plant extracts of Datura stramonium leaves with early stage infections.

Multi-spot, label-free detection of biomarkers in complex media by reflectionless surfaces / M. Salina, F. Giavazzi, E. Ceccarello, F. Damin, M. Chiari, M. Ciuffo, G.P. Accotto, M. Buscaglia. - In: PROCEDIA ENGINEERING. - ISSN 1877-7058. - 87(2014), pp. 58-61. ((Intervento presentato al 28. convegno European Conference on Solid-State Transducers, EUROSENSORS tenutosi a Brescia nel 2014.

Multi-spot, label-free detection of biomarkers in complex media by reflectionless surfaces

M. Salina;F. Giavazzi;E. Ceccarello;M. Buscaglia
2014

Abstract

The measurement of the intensity of light reflected by interfaces with extremely low reflectivity in water enables the label-free, multiplex quantification of the binding between immobilized probes (e.g. antibodies) and targets in solution using extremely simple instrumental set-up. Here we show that, despite its simplicity, the method enables label-free detection in complex samples characterized by high absorbance and turbidity. Diagnostic markers of Tomato spotted wilt virus are revealed in crude plant extracts of Datura stramonium leaves with early stage infections.
Immunoassay; Label-free; Optical biosensor; Reflective phantom interface; Tomato spotted wilt virus; Viral infection
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
PROCEDIA ENGINEERING
Article (author)
File in questo prodotto:
File Dimensione Formato  
PROENG_Eurosensors2014_Buscaglia.pdf

accesso aperto

528.52 kB Adobe PDF Visualizza/Apri
1-s2.0-S1877705814023807-main.pdf

accesso aperto

412.97 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/337321
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact