Background: Ribosomopathies constitute a class of inherited disorders characterized by defects in ribosome biogenesis and function. Classically, bone marrow (BM) failure is a clinical symptom shared between these syndromes, including Shwachman-Bodian-Diamond syndrome (SBDS). Eukaryotic translation initiation factor 6 (eIF6) is a critical translation factor that rescues the quasilethal effect of the loss of the SBDS protein. Objectives: To determine whether eIF6 activity is necessary for BM development. Methods: We used eIF6+/- mice and primary BM megakaryocytes to investigate the involvement of eIF6 in the regulation of hematopoiesis. Results: We provide evidence that reduced eIF6 expression negatively impacts on megakaryopoiesis. We show that inhibition of eIF6 leads to a reduction in cell size and mean ploidy level of megakaryocytes and a delay in megakaryocyte maturation by blocking the G1/S transition. Consistent with this phenotype, only few megakaryocyte-forming proplatelets were found in eIF6+/- cells. We also discovered that, in eIF6+/- cells, the steady-state abundance of mitochondrial respiratory chain complex I-encoding mRNAs is decreased, resulting in decreased reactive oxygen species (ROS) production. Intriguingly, connectivity map analysis showed that eIF6-mediated changes overlap with specific translational inhibitors. eIF6 is a translation factor acting downstream of insulin/phorbol 12-myristate 13-acetate (PMA) stimulation. PMA treatment significantly restored eIF6+/- megakaryocyte maturation, indicating that activation of eIF6 is essential for the rescue of the phenotype. Conclusions: Taken together, our results show a role for eIF6-driven translation in megakaryocyte development, and unveil the novel connection between translational control and ROS production in this cell subset.
Eukaryotic translation initiation factor 6 is a novel regulator of reactive oxygen species-dependent megakaryocyte maturation / S. Ricciardi, A. Miluzio, D. Brina, K. Clarke, M. Bonomo, R. Aiolfi, L.G. Guidotti, F. Falciani, S. Biffo. - In: JOURNAL OF THROMBOSIS AND HAEMOSTASIS. - ISSN 1538-7933. - 13:11(2015 Nov), pp. 2108-2118.
|Titolo:||Eukaryotic translation initiation factor 6 is a novel regulator of reactive oxygen species-dependent megakaryocyte maturation|
MILUZIO, ANNARITA (Secondo)
BIFFO, STEFANO (Ultimo)
|Parole Chiave:||Initiation factors; Megakaryocytes; Platelets; Reactive oxygen species; Thrombocytopenia; Hematology|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||nov-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/jth.13150|
|Appare nelle tipologie:||01 - Articolo su periodico|
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