Microtubules or microtubule bundles in cells often grow longer than the size of the cell, which causes their shape and organization to adapt to constraints imposed by the cell geometry. We test the reciprocal role of elasticity and confinement in the organization of growing microtubules in a con. ning box-like geometry, in the absence of other (active) microtubule organizing processes. This is inspired, for example, by the cortical microtubule array of elongating plant cells, where microtubules are typically organized in an aligned array transverse to the cell elongation axis. The method we adopt is a combination of analytical calculations, in which the polymers are modeled as inextensible. laments with bending elasticity confined to a two-dimensional surface that defines the limits of a three-dimensional space, and in vitro experiments, in which microtubules are polymerized from nucleation seeds in microfabricated chambers. We show that these features are sufficient to organize the polymers in aligned, coiling configurations as for example observed in plant cells. Though elasticity can account for the regularity of these arrays, it cannot account for a transverse orientation of microtubules to the cell's long axis. We therefore conclude that an additional active, force-generating process is necessary to create a coiling configuration perpendicular to the long axis of the cell.

Evaluating the role of elasticity in plant cell cortical microtubule organization : a combined in vitro and modeling approach / M. Cosentino Lagomarsino, C. Tanase, B. Mulder, M. Dogterom, A. Emons, J. Vos. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - 92:3(2007), pp. 1046-1057. [10.1529/biophysj.105.076893]

Evaluating the role of elasticity in plant cell cortical microtubule organization : a combined in vitro and modeling approach

M. Cosentino Lagomarsino
Primo
;
2007

Abstract

Microtubules or microtubule bundles in cells often grow longer than the size of the cell, which causes their shape and organization to adapt to constraints imposed by the cell geometry. We test the reciprocal role of elasticity and confinement in the organization of growing microtubules in a con. ning box-like geometry, in the absence of other (active) microtubule organizing processes. This is inspired, for example, by the cortical microtubule array of elongating plant cells, where microtubules are typically organized in an aligned array transverse to the cell elongation axis. The method we adopt is a combination of analytical calculations, in which the polymers are modeled as inextensible. laments with bending elasticity confined to a two-dimensional surface that defines the limits of a three-dimensional space, and in vitro experiments, in which microtubules are polymerized from nucleation seeds in microfabricated chambers. We show that these features are sufficient to organize the polymers in aligned, coiling configurations as for example observed in plant cells. Though elasticity can account for the regularity of these arrays, it cannot account for a transverse orientation of microtubules to the cell's long axis. We therefore conclude that an additional active, force-generating process is necessary to create a coiling configuration perpendicular to the long axis of the cell.
Plant-cell wall ; preprophase band formation ; cortical microtubules ; self-organization ; microfabricated chambers ; microfibril deposition ; cytoskeleton ; protein ; reorientation ; dynamics
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/32893
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