Process performances of an upflow anaerobic filter treating olive mill wastewater and the response of methanogenic Archaea to increasing volumetric organic load (VOL) were studied. At a VOL of 15 g chemical oxygen demand (COD) L-1 day 90% of the influent COD was removed. Following a VOL increase from 6 to 15 g COD L-1 day(-1), the polymerase chain reaction (PCR) titre of hydrogenotrophic Methanobacterium, determined by magnetic capture of the target DNA and group-specific PCR based on the 16S rRNA gene, decreased from 10(11) to 10(8) cells g(-1) sludge, while that of Methanomicrobiaceae and relatives increased from 10(4) to 10(6) cells g(-1) sludge. Methanosaeta-like acetoclastic methanogens were less affected by VOL variation and dominated at high VOL with a 16S rRNA gene PCR titre of 10(9) cells g(-1) sludge. Single-strand conformation polymorphism analysis of the PCR-amplified archaeal 16S rRNA gene showed a stable band pattern, indicating that VOL variation affected the methanogen PCR titre but not the archaeal community structure. (c) 2006 Society of Chemical Industry.
|Titolo:||Response of methanogen populations to organic load increase during anaerobic digestion of olive mill wastewater|
|Autori interni:||DAFFONCHIO, DANIELE GIUSEPPE|
|Parole Chiave:||Olive mill wastewater ; anaerobic digestion ; methanogens ; organic load ; PCR monitoring ; SSCP|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Data di pubblicazione:||set-2006|
|Appare nelle tipologie:||01 - Articolo su periodico|