Nephroblastorna, a relatively common renal neoplasm Of young swine, represents the aninial counterpart of Wilms' tumour of children. Five porcine nephroblastomas were examined histologically, and immunohistochemically with antibodies against vimentin (VIM), cytokeratins (CKs), smooth-muscle actin, Factor VIII, and laminin. Histologically all showed the three components typical of this tumour: mesenchymal blastema, epithelium (tubuli, and glomeruloid bodies) and stroma. The only antibody recognizing mesenchymal cells was VIM. One-third of tubular structures were positive for VIM. All of the tubules were positive for CK19, two-thirds expressed CK AE1 /AE3, and only one-third expressed CKs 818. Small round tubuli, located in the stromal septa, were positive for CK7 (ureteric branches). Stromal cells expressed both VIM and actin, demonstrating myofibroblastic differentiation. The kidney originates from mesenchymal blastema, which changes to epithelium, losing VIM and acquiring CK expression. In the adult mammalian kidney, CK 19 is expressed only by the parietal epithelium of Bowman's capsule and the distal tubules. Nevertheless, CK19 is also considered a "transient" CK, expressed by different kinds of epithelia during differentiation. CK 19 was also detected in several undifferentiated neoplasins. This Finding, together with the co-expression of VIM detected in some tubules, demonstrates the embryonic origin of nephroblastoma. (c) 2005 Elsevier Ltd. All rights reserved.

Immunohistochemical study of porcine nephroblastoma / V. Grieco, E. Riccardi, S. Belotti, E. Scanziani. - In: JOURNAL OF COMPARATIVE PATHOLOGY. - ISSN 0021-9975. - 134:2-3(2006), pp. 143-151. [10.1016/j.jcpa.2005.09.003]

Immunohistochemical study of porcine nephroblastoma

V. Grieco;E. Riccardi;E. Scanziani
2006

Abstract

Nephroblastorna, a relatively common renal neoplasm Of young swine, represents the aninial counterpart of Wilms' tumour of children. Five porcine nephroblastomas were examined histologically, and immunohistochemically with antibodies against vimentin (VIM), cytokeratins (CKs), smooth-muscle actin, Factor VIII, and laminin. Histologically all showed the three components typical of this tumour: mesenchymal blastema, epithelium (tubuli, and glomeruloid bodies) and stroma. The only antibody recognizing mesenchymal cells was VIM. One-third of tubular structures were positive for VIM. All of the tubules were positive for CK19, two-thirds expressed CK AE1 /AE3, and only one-third expressed CKs 818. Small round tubuli, located in the stromal septa, were positive for CK7 (ureteric branches). Stromal cells expressed both VIM and actin, demonstrating myofibroblastic differentiation. The kidney originates from mesenchymal blastema, which changes to epithelium, losing VIM and acquiring CK expression. In the adult mammalian kidney, CK 19 is expressed only by the parietal epithelium of Bowman's capsule and the distal tubules. Nevertheless, CK19 is also considered a "transient" CK, expressed by different kinds of epithelia during differentiation. CK 19 was also detected in several undifferentiated neoplasins. This Finding, together with the co-expression of VIM detected in some tubules, demonstrates the embryonic origin of nephroblastoma. (c) 2005 Elsevier Ltd. All rights reserved.
nephroblastoma; pig; tumour; Wilms' tumour
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
JOURNAL OF COMPARATIVE PATHOLOGY
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/31078
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 12
social impact