Crown rot is a postharvest fungal disease with a great negative impact on fruit quality. It is caused by several fungal pathogens, including some Fusarium species. We studied Fusarium species associated to crown rot of organic bananas grown in Dominican Republic. Bananas were collected in five organic farms and in the corresponding packing stations over two years. A total of 316 hands were collected and more than 2034 fungal colonies were obtained from crown tissues. Out of them 275 representative Fusarium colonies were purified, characterized and identified. Combinations of two identification keys were used for morphological identification. In addition to, these isolates were characterized by DNA sequencing of nuclear rDNA internal transcribed spacer (ITS), beta-tubulin gene and microsatellite markers. Finally, 11 representative strains were tested for their pathogenicity. Fusarium spp. were isolated from all the analyzed samples from field and packing stations. Moreover they were isolated from different crown layer and also from crown internal tissues. The Fusarium population was composed mainly by seven species and five were the most frequent: F. verticillioides, F. chlamydosporum, F. solani, F. equiseti, and F. oxysporum. F. verticillioides was the most virulent specie which caused the highest disease incidence and severity in experimental pathogenicity tests.
|Titolo:||Morphological and molecular characterization of Fusarium spp. isolated from Crown rot of organic bananas|
KAMEL, MOHAMED ABDALLA MOHAMED (Primo)
SARACCHI, MARCO (Secondo)
CORTESI, PAOLO (Ultimo)
|Parole Chiave:||crown rot; organic bananas; Fusarium spp.; identification; characterization;|
|Settore Scientifico Disciplinare:||Settore AGR/12 - Patologia Vegetale|
|Data di pubblicazione:||ago-2015|
|Enti collegati al convegno:||International Association for the Plant Protection Sciences|
Deutsche Phytomedizinische Gesellschaft e.V.
Julius Kühn-Institut, Federal Research Centre for Cultivated Plants
|Tipologia:||Book Part (author)|
|Appare nelle tipologie:||03 - Contributo in volume|