Two molecular marker approaches [amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR)] were employed to study genomic relationship among 96 rice cultivars. These included most of the best reputed Italian accessions. AFLP produced 461 fragments, 248 (53%) of which were polymorphic, SSR produced four to 11 alleles in the 12 genomic loci investigated. Genomic similarity was estimated independently for the two molecular marker techniques. Both AFLP and SSR dendrograms agree in splitting the cultivars into two main clusters: a small one, comprising four exotic accessions, and a larger one which could be split into four sub-groups. These were also analysed on the basis of historical and pedigree information. This is the first report on the application of DNA polymorphism analysis to reveal genomic relationship among cultivated Italian rice germplasm. Results will be useful for breeding programmes.

Italian rice varieties : hystorical data, molecular markers and pedigrees to reveal their genetic relationships / A. Spada, R. Mantegazza, M. Biloni, E. Caporali, F. Sala. - In: PLANT BREEDING. - ISSN 0179-9541. - 123:2(2004), pp. 105-111.

Italian rice varieties : hystorical data, molecular markers and pedigrees to reveal their genetic relationships

A. Spada;E. Caporali;F. Sala
2004

Abstract

Two molecular marker approaches [amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR)] were employed to study genomic relationship among 96 rice cultivars. These included most of the best reputed Italian accessions. AFLP produced 461 fragments, 248 (53%) of which were polymorphic, SSR produced four to 11 alleles in the 12 genomic loci investigated. Genomic similarity was estimated independently for the two molecular marker techniques. Both AFLP and SSR dendrograms agree in splitting the cultivars into two main clusters: a small one, comprising four exotic accessions, and a larger one which could be split into four sub-groups. These were also analysed on the basis of historical and pedigree information. This is the first report on the application of DNA polymorphism analysis to reveal genomic relationship among cultivated Italian rice germplasm. Results will be useful for breeding programmes.
No
English
Oryza sativa ; AFLP ; genetic similarity ; Italian rice ; molecular markers ; SSR
Settore BIO/01 - Botanica Generale
Articolo
Sì, ma tipo non specificato
Pubblicazione scientifica
2004
Blackwell
123
2
105
111
7
Pubblicato
Periodico con rilevanza internazionale
info:eu-repo/semantics/article
Italian rice varieties : hystorical data, molecular markers and pedigrees to reveal their genetic relationships / A. Spada, R. Mantegazza, M. Biloni, E. Caporali, F. Sala. - In: PLANT BREEDING. - ISSN 0179-9541. - 123:2(2004), pp. 105-111.
reserved
Prodotti della ricerca::01 - Articolo su periodico
5
262
Article (author)
si
A. Spada, R. Mantegazza, M. Biloni, E. Caporali, F. Sala
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/27699
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