In vitro culture of Cimicifuga racemosa

The paper reports the first results of a study on in vitro culture of Cimificuga racemosa. In particular, some experiments were conducted to set up axenic proliferation of shoots from which it could be possible to produce microrhizomes, while in other experiments it was studied the ability of different kinds of explant to develop callus that is needed to initiate a cell suspension culture system. Leaf lamina showed higher competence than petioles in terms of both callus induction and morphogenetic ability. The 2,4-D inductive effect was more remarkable in the dark. Root differentiation of callus occurred only in the dark. Callus growth was generally quite slow and this was likely related to the brownish appearance of the cultures.