Summary During very early stages of flower development in Arabidopsis thaliana, a series of key decisions are taken. Indeed, the position and the basic patterning of new flowers are determined in less than 4 days. Given that the scientific literature provides hard evidence for the function of only 10% of A. thaliana genes, we hypothesized that although many essential genes have already been identified, many poorly characterized genes are likely to be involved in floral patterning. In the current study, we use high-throughput sequencing to describe the transcriptome of the native inflorescence meristem, the floral meristem and the new flower immediately after the start of organ differentiation. We provide evidence that our experimental system is reliable and less affected by experimental artefacts than a widely used floral induction system. Furthermore, we show how these data can be used to identify candidate genes for functional studies, and to generate hypotheses of functional redundancies and regulatory interactions.

Gene coexpression patterns during early development of the native Arabidopsis reproductive meristem : novel candidate developmental regulators and patterns of functional redundancy / O. Mantegazza, V. Gregis, M. Chiara, C. Selva, G. Leo, D.S. Horner, M.M. Kater. - In: PLANT JOURNAL. - ISSN 0960-7412. - 79:5(2014), pp. 861-877. [10.1111/tpj.12585]

Gene coexpression patterns during early development of the native Arabidopsis reproductive meristem : novel candidate developmental regulators and patterns of functional redundancy

O. Mantegazza;V. Gregis;M. Chiara;G. Leo;D.S. Horner;M.M. Kater
2014

Abstract

Summary During very early stages of flower development in Arabidopsis thaliana, a series of key decisions are taken. Indeed, the position and the basic patterning of new flowers are determined in less than 4 days. Given that the scientific literature provides hard evidence for the function of only 10% of A. thaliana genes, we hypothesized that although many essential genes have already been identified, many poorly characterized genes are likely to be involved in floral patterning. In the current study, we use high-throughput sequencing to describe the transcriptome of the native inflorescence meristem, the floral meristem and the new flower immediately after the start of organ differentiation. We provide evidence that our experimental system is reliable and less affected by experimental artefacts than a widely used floral induction system. Furthermore, we show how these data can be used to identify candidate genes for functional studies, and to generate hypotheses of functional redundancies and regulatory interactions.
No
English
Arabidopsis thaliana; Floral meristem; Inflorescence meristem; Laser microdissection; Organ differentiation; RNA sequencing
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
2014
Blackwell Publishing Ltd
79
5
861
877
17
Pubblicato
Periodico con rilevanza internazionale
scopus
pubmed
crossref
Aderisco
info:eu-repo/semantics/article
Gene coexpression patterns during early development of the native Arabidopsis reproductive meristem : novel candidate developmental regulators and patterns of functional redundancy / O. Mantegazza, V. Gregis, M. Chiara, C. Selva, G. Leo, D.S. Horner, M.M. Kater. - In: PLANT JOURNAL. - ISSN 0960-7412. - 79:5(2014), pp. 861-877. [10.1111/tpj.12585]
reserved
Prodotti della ricerca::01 - Articolo su periodico
7
262
Article (author)
si
O. Mantegazza, V. Gregis, M. Chiara, C. Selva, G. Leo, D.S. Horner, M.M. Kater
File in questo prodotto:
File Dimensione Formato  
Mantegazza_et_al-2014-The_Plant_Journal.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.21 MB
Formato Adobe PDF
1.21 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/255396
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 23
social impact