The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the murB gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity.

Purification and partial characterization of a novel bacteriocin produced by a thermophilic endospore-forming strain Geobacillus stearothermophilus 32A / K. Pokusajeva, M. Stuknyte, N. Kuisiene, D. Chitavichius. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 222:suppl. 1(2003 Jun 29), pp. 107-107. ((Intervento presentato al 1. convegno FEMS Congress of European Microbiologists tenutosi a Ljubljana nel 2003 [10.1016/S0378-1097(03)00297-0].

Purification and partial characterization of a novel bacteriocin produced by a thermophilic endospore-forming strain Geobacillus stearothermophilus 32A

M. Stuknyte;
2003

Abstract

The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the murB gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity.
Settore AGR/15 - Scienze e Tecnologie Alimentari
Settore AGR/16 - Microbiologia Agraria
Settore BIO/10 - Biochimica
Settore BIO/11 - Biologia Molecolare
Settore BIO/19 - Microbiologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/254737
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