Horizontalios genu pernasos galimybe Geobacillus genties bakterijose=Possibility of horizontal gene transfer in the genus Geobacillus

Horizontal gene transfbr determines bacterial adaptation to changing environment. The principal means to achieve t[ris is conjugative plasmid transfer. Structural and functional orgarrization of conjugative transfer apparatus in Gram-negative bacteria Escherichia coli and Agrobaaerium tumefac,zszsis well-known. ln contrary, information in this field concerning Grampositive bacteria is not systematized, more studied only in a cotrple of genera: Bacilhrs, Enterococcas. Tlreir conjugativc plasmids in erddition to origin of transfcr oriTutdgenes, encoding DNA relaxase, carry also Ti plasmid homologs of A. rumefacienrYrcBl 1 and VirB4 adenosine triphosphatases, VirB11 lytic transglycosilases, YirD4 coupling protcins. Up to date, conjugation possibilities in the genus Geobacillu'tre not analyzcd at all. Accomplishnrent of partial sequence analysis of the plasrnid from G. stearoilterrnophiltu 18 allowed us to identify *o open reading frarrres rhat potential gene products are homologous to Lactococcus lactisTraD a:nd Lactobacillus partrcasaisul:sp.arrtcasaiTrtb,protcins associated with horizotrtalgene transfer. These findings and future homology srudies with the othcr plasnrids from geobacilli will have an important impact on understanding of horizontal gene transfer evcnts in dris genus.