The effect of arachidonic acid (AA) on intracellular Ca(2+) concentration ([Ca(2+)]i) in human osteoblasts MG63 was studied. AA caused a concentration-dependent increase in [Ca(2+)]i, mainly due to inward Ca(2+) transport from extracellular environment. Moreover, AA in Ca(2+) -free medium produced a small, transient increase of [Ca(2+)]i, indicating that AA may also trigger Ca(2+) release from intracellular stores. Because the [Ca(2+)]i response to AA was inhibited by the cyclooxygenase (COX) inhibitor indomethacin, we tested the effect of prostaglandins (PGs), products of COX pathway. PGs E1 and E2 caused an increase in [Ca(2+)]i, which, however, was far lower than that obtained with AA. The [Ca(2+)]i response to AA was not inhibited by nifedipine, suggesting that AA did not activate a voltage-dependent Ca(2+) channel. Our results indicate that AA could modulate [Ca(2+)]i in MG63 human osteoblasts, where it may influence Ca(2+) transport across both plasma and endoplasmic membranes. Furthermore, they suggest that osteoblast activity may be modulated by AA.
Arachidonic acid influences intracellular calcium handling in human osteoblasts / L. Soldati, A. Terranegra, B. Baggio, R. Biasion, T. Arcidiacono, G. Priante, D. Cusi, G. Vezzoli. - In: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS. - ISSN 0952-3278. - 75:2(2006 Aug), pp. 91-96.
|Titolo:||Arachidonic acid influences intracellular calcium handling in human osteoblasts|
SOLDATI, LAURA (Primo)
TERRANEGRA, ANNALISA (Secondo)
CUSI, DANIELE MARIA (Penultimo)
|Settore Scientifico Disciplinare:||Settore MED/14 - Nefrologia|
|Data di pubblicazione:||ago-2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.plefa.2006.05.015|
|Appare nelle tipologie:||01 - Articolo su periodico|