Lupin is a protein-rich grain legume, which provide some health benefits, particularly in the area of dislipidemia prevention. Previous experiments in suitable animal models and in mild hypercholesterolemic individuals have shown that the consumption of lupin proteins may be useful for controlling total and low-density-lipoprotein (LDL) cholesterol levels [1-2]. With the objective of providing evidences that peptides generated by the digestion of lupin proteins may be responsible of the observed activities and for investigating the mechanism of action, HepG2 cells were treated with lupin peptides obtained either by pepsin (P) or trypsin (T) hydrolysis and molecular and functional investigations were performed on the LDL receptor / SREBP2 pathway. For the first time, this report provides the experimental evidence that peptides obtained from the hydrolysis of lupin proteins are able to interfere with the HMGCoAR activity, up-regulating the LDL receptor and SREBP2 proteins via the activation of PI3K/Akt/GSK3β pathways and increasing the LDL-uptake at HepG2 cell line. [1] M. Marchesi, C. Parolini, E. Diani, E. Rigamonti, L. Cornelli, A. Arnoldi, CR Sirtori, G. Chiesa. Brit. J Nutr., 2008, 100, 707-10. [2] C.R. Sirtori, M. Triolo, R .Bosisio, A. Bondioli, L. Calabresi , V. De Vergori, M. Gomaraschi, G. Mombelli, F. Pazzucconi, C. Zacherl, A. Arnoldi Brit. J Nutr., 2012, 107, 1176-83.
Lupin peptides induce the LDL receptor / SREBP2 pathway at HEPG2 cell line / C. Lammi, C. Zanoni, G.M. Scigliuolo, A. D’Amato, A. Arnoldi - In: XXV Congresso Nazionale della Società Chimica Italiana[s.l] : Società Chimica Italiana, 2014. - pp. 375-375 (( Intervento presentato al 25. convegno Congresso Nazionale Società Chimica Italiana (SCI) tenutosi a Arcavacata di Rende nel 2014.
Lupin peptides induce the LDL receptor / SREBP2 pathway at HEPG2 cell line
C. LammiPrimo
;C. ZanoniSecondo
;A. D’Amato;A. ArnoldiUltimo
2014
Abstract
Lupin is a protein-rich grain legume, which provide some health benefits, particularly in the area of dislipidemia prevention. Previous experiments in suitable animal models and in mild hypercholesterolemic individuals have shown that the consumption of lupin proteins may be useful for controlling total and low-density-lipoprotein (LDL) cholesterol levels [1-2]. With the objective of providing evidences that peptides generated by the digestion of lupin proteins may be responsible of the observed activities and for investigating the mechanism of action, HepG2 cells were treated with lupin peptides obtained either by pepsin (P) or trypsin (T) hydrolysis and molecular and functional investigations were performed on the LDL receptor / SREBP2 pathway. For the first time, this report provides the experimental evidence that peptides obtained from the hydrolysis of lupin proteins are able to interfere with the HMGCoAR activity, up-regulating the LDL receptor and SREBP2 proteins via the activation of PI3K/Akt/GSK3β pathways and increasing the LDL-uptake at HepG2 cell line. [1] M. Marchesi, C. Parolini, E. Diani, E. Rigamonti, L. Cornelli, A. Arnoldi, CR Sirtori, G. Chiesa. Brit. J Nutr., 2008, 100, 707-10. [2] C.R. Sirtori, M. Triolo, R .Bosisio, A. Bondioli, L. Calabresi , V. De Vergori, M. Gomaraschi, G. Mombelli, F. Pazzucconi, C. Zacherl, A. Arnoldi Brit. J Nutr., 2012, 107, 1176-83.File | Dimensione | Formato | |
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