Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins γ1, β1, μ1, σ1. Mammalia express μ1A ubiquitously and isoform μ1B in polarized epithelia. Mouse γ1 or μ1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated μ1A and μ1B from Danio rerio. Analysis of μ1A and μ1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. μ1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. μ1B is not expressed in murine liver, indicating loss of μ1B expression and establishment of alternative sorting mechanisms during mammalian development.

Characterization of the AP-1 μ1A and μ1B adaptins in zebrafish (Danio rerio) / D. Zizioli, E. Forlanelli, M. Guarienti, S. Nicoli, A. Fanzani, R. Bresciani, G. Borsani, A. Preti, F. Cotelli, P. Schu. - In: DEVELOPMENTAL DYNAMICS. - ISSN 1058-8388. - 239:9(2010 Sep), pp. 2404-2412. [10.1002/dvdy.22372]

Characterization of the AP-1 μ1A and μ1B adaptins in zebrafish (Danio rerio)

F. Cotelli;
2010

Abstract

Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins γ1, β1, μ1, σ1. Mammalia express μ1A ubiquitously and isoform μ1B in polarized epithelia. Mouse γ1 or μ1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated μ1A and μ1B from Danio rerio. Analysis of μ1A and μ1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. μ1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. μ1B is not expressed in murine liver, indicating loss of μ1B expression and establishment of alternative sorting mechanisms during mammalian development.
Adaptor protein complexes; AP-1; Clathrin; Gut; Membrane traffic; Trans-golgi network; Zebrafish
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/238642
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