A disposable electrochemical biosensor for the determination of L-DOPA in undiluted human serum is described. It is based on the use of a carbon nanotubes-modified screen printed electrode covered with an electropolymerized poly(thionine) film and an outer cross-linked layer containing tyrosinase. Further coverage of the sensor with a Nafion layer allows its use directly in human serum, obviating the need for sample pretreatment. The sensor exhibited a linear behavior in the 0.8–22 μM concentration range and offers promise for decentralized clinical testing of L-DOPA.
A disposable electrochemical biosensor for l-DOPA determination in undiluted human serum / B. Brunetti, G. Valdés-Ramírez, I. Litvan, J. Wang. - In: ELECTROCHEMISTRY COMMUNICATIONS. - ISSN 1388-2481. - 48(2014), pp. 28-31. [10.1016/j.elecom.2014.08.007]
A disposable electrochemical biosensor for l-DOPA determination in undiluted human serum
B. Brunetti
;
2014
Abstract
A disposable electrochemical biosensor for the determination of L-DOPA in undiluted human serum is described. It is based on the use of a carbon nanotubes-modified screen printed electrode covered with an electropolymerized poly(thionine) film and an outer cross-linked layer containing tyrosinase. Further coverage of the sensor with a Nafion layer allows its use directly in human serum, obviating the need for sample pretreatment. The sensor exhibited a linear behavior in the 0.8–22 μM concentration range and offers promise for decentralized clinical testing of L-DOPA.
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