Most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. The aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. The list of targets include, among the others, Listeria spp., Salmonella spp., Shigella spp., Escherichia coli spp., Campylobacter spp., Clostridium spp. and Staphylococcus aureus. The accuracy of detection was determined by using ATCC strains as positive and negative controls. The achieved sensitivity of each of assays was 1pg of genomic DNA, which was equivalent to ~1cfu. The working ranges of the TaqMan® Real-time PCR assays, when used quantitatively on cheese and meat samples inoculated with serial dilution of Listeria spp., Listeria monocytogenes, S.aureus, Salmonella enterica, Shigella boydii, E.coli O157:H7, Bacillus cereus, Campylobacter coli, Yersinia enterocolitica, Enterobacter sakazakii and Pseudomonas aeruginosa was 108cfu/g to 104cfu/g. No matrix interferences were observed. © 2014 Elsevier Ltd.

Development of 23 individual TaqMan® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions / P. Cremonesi, L.F. Pisani, C. Lecchi, F. Ceciliani, P. Martino, A.S. Bonastre, A. Karus, C. Balzaretti, B. Castiglioni. - In: FOOD MICROBIOLOGY. - ISSN 0740-0020. - 43(2014), pp. 35-40. [10.1016/j.fm.2014.04.007]

Development of 23 individual TaqMan® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions

L.F. Pisani
Secondo
;
C. Lecchi;F. Ceciliani;P. Martino;C. Balzaretti
Penultimo
;
2014

Abstract

Most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. The aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. The list of targets include, among the others, Listeria spp., Salmonella spp., Shigella spp., Escherichia coli spp., Campylobacter spp., Clostridium spp. and Staphylococcus aureus. The accuracy of detection was determined by using ATCC strains as positive and negative controls. The achieved sensitivity of each of assays was 1pg of genomic DNA, which was equivalent to ~1cfu. The working ranges of the TaqMan® Real-time PCR assays, when used quantitatively on cheese and meat samples inoculated with serial dilution of Listeria spp., Listeria monocytogenes, S.aureus, Salmonella enterica, Shigella boydii, E.coli O157:H7, Bacillus cereus, Campylobacter coli, Yersinia enterocolitica, Enterobacter sakazakii and Pseudomonas aeruginosa was 108cfu/g to 104cfu/g. No matrix interferences were observed. © 2014 Elsevier Ltd.
Cheese ; foodborne pathogens ; meat ; TaqMan(®) real-time quantitative PCR
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/236550
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