In vitro incubation with human liver microsomes of JWH-018, JWH-073, JWH-122 and for the first time 1-butyl-3-(1-(4-methyl)naphthoyl)indole (the 4-methylnaphthoyl analogue of JWH-073) was investigated to identify the principal metabolites of alkylindole synthetic cannabinoids, thus helping the discovering of synthetic cannabinoids abusers. The results obtained showed that the most abundant metabolites were mono-hydroxylated derivatives either on the alkyl chain (ω or ω-1 position) or on the indole (presumably in position 5 or 6) and naphthalene moieties.Moreover the extraction conditions of these derivatives from biological fluids, mainly plasma and urine spiked with commercially available metabolite standards, and the incubation procedure were investigated to obtain a fast, reliable and suitable extraction protocol to detect either the parent drugs or their metabolites by means of GC/MS.
Characterization of in vitro metabolites of JWH-018, JWH-073 and their 4-methyl derivatives, markers of the abuse of these synthetic cannabinoids / V. Gambaro, S. Arnoldi, S. Bellucci, E. Casagni, L. Dell'acqua, L. Fumagalli, M. Pallavicini, G. Roda, C. Rusconi, E. Valoti. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 957(2014 Apr 15), pp. 68-76.
|Titolo:||Characterization of in vitro metabolites of JWH-018, JWH-073 and their 4-methyl derivatives, markers of the abuse of these synthetic cannabinoids|
|Parole Chiave:||Alkylindole metabolites; GC/FID; GC/MS; JWH-018; JWH-073; JWH-122 ;1-Butyl-3-(1-(4-methyl)naphthoyl)indole|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
|Data di pubblicazione:||15-apr-2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jchromb.2014.03.001|
|Appare nelle tipologie:||01 - Articolo su periodico|