Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.
Quantitative measurement of blood velocity in zebrafish with optical vector field tomography / L. Fieramonti, E.A. Foglia, S. Malavasi, C. D'Andrea, G. Valentini, F. Cotelli, A. Bassi. - In: JOURNAL OF BIOPHOTONICS. - ISSN 1864-063X. - 8:1/2(2015 Jan), pp. 52-59. [10.1002/jbio.201300162]
Quantitative measurement of blood velocity in zebrafish with optical vector field tomography
E.A. FogliaSecondo
;F. CotelliPenultimo
;
2015
Abstract
Microscopy techniques can readily visualize the finest details of embryo vasculature, but still lack to provide a complete three-dimensional representation of blood flow parameters. We present an in-vivo 3D imaging technique, able to reconstruct the blood cell velocity vector over a large volume of zebrafish embryos. This low cost and relatively simple technique is exploited to quantitatively assess blood velocity in the zebrafish tail at different stages of development.File | Dimensione | Formato | |
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