The High-Mobility Group Box (HMGB) proteins are highly abundant proteins with both nuclear and extracellular roles in key biological processes. In mammals, three family members are present: HMGB1, HMGB2 and HMGB3. We characterized the HMGB family in zebrafish and report a detailed phylogenetic analysis of HMGB proteins. The B1, B2, and B3 subfamilies are present in cartilaginous fish, bony fish, and tetrapods, while jawless fish sequences emerge as basal to the gene family expansion. Two co-orthologs of each mammalian HMGB gene are present in zebrafish. All six zebrafish hmgb genes are maternally expressed, but huge differences in expression levels exist during embryonic development. The hmgb2a/hmgb2b genes are the most highly expressed, while hmgb3b is expressed at the lowest level. Remarkably, hmgb3 genes are not present in fugu, medaka, Tetraodon and stickleback. Our analysis highlights substantial overlaps, but also subtle differences and specificities in the expression patterns of the zebrafish hmgb genes.

The HMGB protein gene family in zebrafish : evolution and embryonic expression patterns / S. Moleri, G. Cappellano, G. Gaudenzi, S. Cermenati, F. Cotelli, D.S. Horner, M. Beltrame. - In: GENE EXPRESSION PATTERNS. - ISSN 1567-133X. - 11:1/2(2011), pp. 3-11. [10.1016/j.gep.2010.08.006]

The HMGB protein gene family in zebrafish : evolution and embryonic expression patterns

S. Moleri;G. Cappellano;G. Gaudenzi;S. Cermenati;F. Cotelli;D.S. Horner;M. Beltrame
2011

Abstract

The High-Mobility Group Box (HMGB) proteins are highly abundant proteins with both nuclear and extracellular roles in key biological processes. In mammals, three family members are present: HMGB1, HMGB2 and HMGB3. We characterized the HMGB family in zebrafish and report a detailed phylogenetic analysis of HMGB proteins. The B1, B2, and B3 subfamilies are present in cartilaginous fish, bony fish, and tetrapods, while jawless fish sequences emerge as basal to the gene family expansion. Two co-orthologs of each mammalian HMGB gene are present in zebrafish. All six zebrafish hmgb genes are maternally expressed, but huge differences in expression levels exist during embryonic development. The hmgb2a/hmgb2b genes are the most highly expressed, while hmgb3b is expressed at the lowest level. Remarkably, hmgb3 genes are not present in fugu, medaka, Tetraodon and stickleback. Our analysis highlights substantial overlaps, but also subtle differences and specificities in the expression patterns of the zebrafish hmgb genes.
high-mobility group; high-mobility group box; gene family; phylogenetic analysis; molecular evolution; gene expression; development; embryonic development; vertebrates; fish; danio rerio; in situ hybridization; synteny; chromatin; transcription; recombination; repair; inflammation; innate immunity; tissue regeneration
Settore BIO/11 - Biologia Molecolare
Settore BIO/18 - Genetica
Settore BIO/06 - Anatomia Comparata e Citologia
GENE EXPRESSION PATTERNS
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S1567133X10000852-main.pdf

non disponibili

1.62 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/233232
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 26
social impact