Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were [lambda] = 1.314 [mu]m and [tau] (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I [greater-than-or-equal] 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.

X-ray microscopy of living multicellular organisms with the Prague asterix iodine laser system / T. Desai, D. Batani, A. Bernardinello, G. Poletti, F. Orsini, J. Ullschmied, L. Juha, J. Skala, B. Kralikova, E. Krousky, M. Pfeifer, C. Kadlec, T. Mocek, A. Präg, O. Renner, F. Cotelli, C.L. Lamia, A. Zullini. - In: LASER AND PARTICLE BEAMS. - ISSN 0263-0346. - 21:4(2003), pp. 511-516.

X-ray microscopy of living multicellular organisms with the Prague asterix iodine laser system

G. Poletti;F. Orsini;F. Cotelli;
2003

Abstract

Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were [lambda] = 1.314 [mu]m and [tau] (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I [greater-than-or-equal] 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.
Atomic force microscopy; Laser-produced plasmas; Multicellular Caenorhabditis elegans; Soft X-ray contact microscopy
Settore FIS/03 - Fisica della Materia
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore FIS/01 - Fisica Sperimentale
2003
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/233086
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