1,2-Dichloroethane (1,2-DCA) is a chlorinated compound extensively employed in industrial processes, primarily as an intermediate for PVC production. The widespread use of this compound have led to serious environmental problems in industrialized countries. Several halorespiring bacteria have been identified and cultured, able to use halogenated hydrocarbons as terminal electron acceptors for anaerobic respiration. So far nevertheless, only a few bacterial species with the ability of converting 1,2-DCA into ethene have been identified, mainly belonging to the order Clostridiales. The aim of this work was to identify and enrich bacteria directly involved in the dehalorespiration process from groundwaters historically polluted by high levels of 1,2-DCA. A series of subsequent culture dilutions were established in microcosms, where the microbial community of the aquifer was grown in mineral media supplemented with 1,2-DCA as electron acceptor and lactate or acetate+formate as electron donors. 1,2-DCA degradation was monitored by head-space gas-chromatography. All microcosms showed dehalogenating activity with degradation rates up to 40 ppm 1,2-DCA day-1. Bacterial phylogenetic diversity was monitored by different 16S rRNA gene-based molecular approaches such as PCR-Denaturing Gradient Gel Electrophoresis, barcoded pyrosequencing and fluorescent in situ hybridization. The results demonstrated a gradual simplification of the bacterial community in microcosms supplied with electron donors resulting in the enrichment of few bacterial species, potentially involved in the contaminant depletion, interestingly not belonging to Clostridiales, but to γ- and δ-Proteobacteria.

Enrichment and identification of new putative dehalorespiring bacteria able to degrade 1,2-dichloroethane / G. Merlino, M. Barbato, F. Mapelli, D. Lavazza, S. Borin, G. Carpani, E. Vitale, F. de Ferra, D. Daffonchio. ((Intervento presentato al convegno ULIXES International Conference MedRem - Microbial resource management for polluted marine environments and bioremediation tenutosi a Hamammet, Tunisia nel 2014.

Enrichment and identification of new putative dehalorespiring bacteria able to degrade 1,2-dichloroethane

G. Merlino
Primo
;
M. Barbato
Secondo
;
F. Mapelli;D. Lavazza;S. Borin;D. Daffonchio
Ultimo
2014

Abstract

1,2-Dichloroethane (1,2-DCA) is a chlorinated compound extensively employed in industrial processes, primarily as an intermediate for PVC production. The widespread use of this compound have led to serious environmental problems in industrialized countries. Several halorespiring bacteria have been identified and cultured, able to use halogenated hydrocarbons as terminal electron acceptors for anaerobic respiration. So far nevertheless, only a few bacterial species with the ability of converting 1,2-DCA into ethene have been identified, mainly belonging to the order Clostridiales. The aim of this work was to identify and enrich bacteria directly involved in the dehalorespiration process from groundwaters historically polluted by high levels of 1,2-DCA. A series of subsequent culture dilutions were established in microcosms, where the microbial community of the aquifer was grown in mineral media supplemented with 1,2-DCA as electron acceptor and lactate or acetate+formate as electron donors. 1,2-DCA degradation was monitored by head-space gas-chromatography. All microcosms showed dehalogenating activity with degradation rates up to 40 ppm 1,2-DCA day-1. Bacterial phylogenetic diversity was monitored by different 16S rRNA gene-based molecular approaches such as PCR-Denaturing Gradient Gel Electrophoresis, barcoded pyrosequencing and fluorescent in situ hybridization. The results demonstrated a gradual simplification of the bacterial community in microcosms supplied with electron donors resulting in the enrichment of few bacterial species, potentially involved in the contaminant depletion, interestingly not belonging to Clostridiales, but to γ- and δ-Proteobacteria.
17-gen-2014
Bioremediation, reductive dechlorination, 1,2-dichloroethane, PCR-DGGE, pyrosequencing
Settore AGR/16 - Microbiologia Agraria
Enrichment and identification of new putative dehalorespiring bacteria able to degrade 1,2-dichloroethane / G. Merlino, M. Barbato, F. Mapelli, D. Lavazza, S. Borin, G. Carpani, E. Vitale, F. de Ferra, D. Daffonchio. ((Intervento presentato al convegno ULIXES International Conference MedRem - Microbial resource management for polluted marine environments and bioremediation tenutosi a Hamammet, Tunisia nel 2014.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/231123
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