We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for discrimination between molecular types of Cryptococcus neoformans and Cryptococcus gattii / B. Posteraro, A. Vella, M. Cogliati, E. De Carolis, A.R. Florio, P. Posteraro, M. Sanguinetti, A.M. Tortorano. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 50:7(2012 Jul), pp. 2472-2476. [10.1128/JCM.00737-12]

Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for discrimination between molecular types of Cryptococcus neoformans and Cryptococcus gattii

M. Cogliati;A.M. Tortorano
Ultimo
2012

Abstract

We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.
Cryptococcosis; Cryptococcus gattii; Cryptococcus neoformans; Humans; Microbiological Techniques; Mycology; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors
Settore MED/42 - Igiene Generale e Applicata
lug-2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/226881
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