An integrated proteomic anbd metabolomic approach for the study of exocarp in four grape cultivars with different anthocyanin content

The great diffusion of techniques relating to high-throughput analysis, as well as the availability of a complete and high quality draft of the genome sequence of Vitis vinifera, allowed to deepen the knowledge of grape berry development, necessary assumption in order to satisfy the new and exigent market claims on product quality. Through recent transcriptomic and proleomic studies it was shown that the impact of many sources of variability (e.g. genetical and environmental) during grape development was underrated, leading to badly generalize the results. A faclor that surely deeply contributes in distinguishing the composition 01 the cultivars is the effect of Ihe biosynthesis relating to secondary metabolism during the ripening. In order to evaluate this aspect, we conducted a ,comparative analysis among four vines characterized by different anthocyanin content both at the proteomic and metabolomic level. We decided to locus on mature berry skin, which is the tissue regarded as the richest as far as it concerns secondary metabolism. The integration of the results deriving from 2-DE gels LC-ESI-MS/MS and GC-MS chromatograms relative lo !WO subsequent vintages, emphasized how the differences in the extent of anthocyanin biosynthesis contribute in markedly influence relevant traits glycolysis, Krebs cycle and amino acid metabolism.