During mouse skeletal muscle development, the Nfix gene has a pivotal role in regulating fetal-specific transcription. Zebrafish and mice share related programs for muscle development, although zebrafish develops at a much faster rate. In fact, although mouse fetal muscle fibers form after 15 days of development, in fish secondary muscle fibers form by 48 hours post-fertilization in a process that until now has been poorly characterized mechanically. In this work, we studied the zebrafish ortholog Nfix (nfixa) and its role in the proper switch to the secondary myogenic wave. This allowed us to highlight evolutionarily conserved and divergent functions of Nfix. In fact, the knock down of nfixa in zebrafish blocks secondary myogenesis, as in mouse, but also alters primary slow muscle fiber formation. Moreover, whereas Nfix mutant mice are motile, nfixa knockdown zebrafish display impaired motility that probably depends upon disruption of the sarcoplasmic reticulum. We conclude that, during vertebrate evolution, the transcription factor Nfix lost some specific functions, probably as a consequence of the different environment in which teleosts and mammals develop.

Conserved and divergent functions of Nfix in skeletal muscle development during vertebrate evolution / A. Pistocchi, G. Gaudenzi, E. Foglia, S. Monteverde, A. Moreno Fortuny, A. Pianca, G. Cossu, F. Cotelli, G. Messina. - In: DEVELOPMENT. - ISSN 0950-1991. - 140:7(2013 Apr), pp. 1528-1536. [10.1242/dev.076315]

Conserved and divergent functions of Nfix in skeletal muscle development during vertebrate evolution

A. Pistocchi
Primo
;
G. Gaudenzi
Secondo
;
E. Foglia;S. Monteverde;G. Cossu;F. Cotelli
Penultimo
;
G. Messina
2013

Abstract

During mouse skeletal muscle development, the Nfix gene has a pivotal role in regulating fetal-specific transcription. Zebrafish and mice share related programs for muscle development, although zebrafish develops at a much faster rate. In fact, although mouse fetal muscle fibers form after 15 days of development, in fish secondary muscle fibers form by 48 hours post-fertilization in a process that until now has been poorly characterized mechanically. In this work, we studied the zebrafish ortholog Nfix (nfixa) and its role in the proper switch to the secondary myogenic wave. This allowed us to highlight evolutionarily conserved and divergent functions of Nfix. In fact, the knock down of nfixa in zebrafish blocks secondary myogenesis, as in mouse, but also alters primary slow muscle fiber formation. Moreover, whereas Nfix mutant mice are motile, nfixa knockdown zebrafish display impaired motility that probably depends upon disruption of the sarcoplasmic reticulum. We conclude that, during vertebrate evolution, the transcription factor Nfix lost some specific functions, probably as a consequence of the different environment in which teleosts and mammals develop.
nfix; sarcoplasmic reticulum; skeletal myogenesis; slow muscle fibers; zebrafish; protein nfix; transcription factor; unclassified drug
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/222994
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