Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL1 is a short sequence transferred to the 5' end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.

Unusual organization of the 5S ribosomal spacer in Dirofilaria repens : absence of a canonical spliced leader 1 sequence / G. Favia, C. Bazzocchi, G. Cancrini, C. Genchi, C. Bandi. - In: PARASITOLOGY RESEARCH. - ISSN 0932-0113. - 86:6(2000 Jun), pp. 497-499.

Unusual organization of the 5S ribosomal spacer in Dirofilaria repens : absence of a canonical spliced leader 1 sequence

G. Favia
Primo
;
C. Bazzocchi
Secondo
;
C. Genchi
Penultimo
;
C. Bandi
Ultimo
2000-06

Abstract

Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL1 is a short sequence transferred to the 5' end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/222669
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