We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species. (C) 2000 Academic Press.

5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications / G. Favia, G. Cancrini, I. Ricci, C. Bazzocchi, M. Magi, M. Pietrobelli, C. Genchi, C. Bandi. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 14:5(2000 Oct), pp. 285-290. [10.1006/mcpr.2000.0317]

5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications

G. Favia
Primo
;
C. Bazzocchi;C. Genchi
Penultimo
;
C. Bandi
Ultimo
2000

Abstract

We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species. (C) 2000 Academic Press.
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
ott-2000
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/222663
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 16
social impact