We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species. (C) 2000 Academic Press.

5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications / G. Favia, G. Cancrini, I. Ricci, C. Bazzocchi, M. Magi, M. Pietrobelli, C. Genchi, C. Bandi. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 14:5(2000 Oct), pp. 285-290.

5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications

G. Favia
Primo
;
C. Bazzocchi;C. Genchi
Penultimo
;
C. Bandi
Ultimo
2000-10

Abstract

We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species. (C) 2000 Academic Press.
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/222663
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