We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species. (C) 2000 Academic Press.
5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications / G. Favia, G. Cancrini, I. Ricci, C. Bazzocchi, M. Magi, M. Pietrobelli, C. Genchi, C. Bandi. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 14:5(2000 Oct), pp. 285-290.
Titolo: | 5 S ribosomal spacer sequences of some filarial parasites : comparative analysis and diagnostic applications |
Autori: | FAVIA, GUIDO (Primo) GENCHI, CLAUDIO (Penultimo) BANDI, CLAUDIO (Ultimo) |
Settore Scientifico Disciplinare: | Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali |
Data di pubblicazione: | ott-2000 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1006/mcpr.2000.0317 |
Appare nelle tipologie: | 01 - Articolo su periodico |