Antibodies raised against human a2-6 and b2-4 nicotinic receptor subunits were utilized to fractionate 3H-epibatidine binding in human temporal cortex and striatum. The predominant receptor subtypes in both regions contained a4 and b2 subunits. In normal cortex, 10% of binding was also assocd. with a2 subunits, whereas in the striatum, contributions by a6 (17%) and b3 (23%) were obsd. Minimal binding (?5%) was assocd. with a3. In Alzheimer's disease and dementia with Lewy bodies, cortical loss of binding was assocd. with redns. in a4 (50%, P < 0.01) and b2 (30-38%, P < 0.05). In Parkinson's disease and dementia with Lewy bodies, striatal deficits in a6 (91 and 59% resp., P < 0.01) and b3 (72 and 75%, P < 0.05) tended to be greater than for a4 and b2 (50-58%, P < 0.05). This study demonstrates distinct combinations of subunits contributing to heteromeric nicotinic receptor binding in the human brain that are area/pathway specific and differentially affected by neurodegeneration. [on SciFinder (R)]
Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer's disease, Parkinson's disease and dementia with Lewy bodies by immunoprecipitation / Cecilia Gotti, Milena Moretti, Iwo Bohr, Iryna Ziabreva, Silvia Vailati, Renato Longhi, Loredana Riganti, Annalisa Gaimarri, Ian G. McKeith, Robert H. Perry, Dag Aarsland, Jan Petter Larsen, Emanuele Sher, Ruth Beattie, Francesco Clementi, Jennifer A. Court. - In: NEUROBIOLOGY OF DISEASE. - ISSN 0969-9961. - 23:2(2006 Aug), pp. 481-489.
|Titolo:||Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer's disease, Parkinson's disease and dementia with Lewy bodies by immunoprecipitation|
|Parole Chiave:||αBungarotoxin; Alzheimer's disease; Choline acetyltransferase; Dementia with Lewy bodies; Epibatidine; Human brain; Immunoprecipitation; Nicotinic acetylcholine receptors; Parkinson's disease; Subunits|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ago-2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.nbd.2006.04.005|
|Appare nelle tipologie:||01 - Articolo su periodico|