Micropropagation of service tree (Sorbus domestica L.) : role of some factors on in vitro proliferation and rooting, and extra vitro acclimatization

The use of in vitro culture, as a tool of vegetative propagation, contributes to both large scale multiplications of interesting genotypes. Regards explant establishment 100% of both sterile and proliferating explants were obtained cutting the explants from two years old micropropagated donor plants. Two selected lines out of three were stimulated in proliferation from the highest (5.0 ?M) BA concentration. 1000 g l-1 IBA and 2.5 ?M BA enhanced rooting in two selected lines out of three. Regarding extra vitro acclimatization the importance of growing media is often overlooked in scientific reports. Different types of growing media were tested, and the relationships among their chemical and physical properties and plant responses were investigated.