Characterization of Lactobacillus helveticus strains isolated from cheeses by distribution studies of insertion sequences

A collection of 38 Lactobacillus helveticus strains, isolated from a no. of different artisan Italian cheeses, and 4 ref. strains were studied with respect to the presence of insertion sequences and their distribution and abundance. The mobile genetic element ISLh1, that contains one open reading frame coding for a putative transposase of the IS982 family, was used for DNA fingerprinting, together with IS1201 and ISL2, previously isolated from L. helveticus. The no. of insertion sequences per strain and the size of DNA restriction fragments contg. them, was variable and allowed the discrimination at the strain-level. The genomic distribution of the three unrelated insertion sequences showed significant correlations and allowed the differentiation of the strains also with regard to the specific ecol. niche of origin of the isolates. Consequently, insertion sequences comparison may be useful in detg. the history of a group of strains known to be related because of identity and offers a further parameter for evaluating the population polymorphism in L. helveticus. [on SciFinder (R)]