This study investigated whether the antiinflammatory effect of chamomile infusion at gastric level could be ascribed to the inhibition of metalloproteinase-9 and elastase. The infusions from capitula and sifted flowers (250–1500mg/mL) and individual flavonoids (10mM) were tested on phorbol 12-myristate 13-acetate-stimulated AGS cells and human neutrophil elastase. The results indicate that the antiinflammatory activity associated with chamomile infusions from both the capitula and sifted flowers is most likely due to the inhibition of neutrophil elastase and gastric metalloproteinase-9 activity and secretion; the inhibition occurring in a concentration dependent manner. The promoter activity was inhibited as well and the decrease of metalloproteinase-9 expression was found to be associated with the inhibition of NF-kB driven transcription. The results further indicate that the flavonoid-7-glycosides, major constituents of chamomile flowers, may be responsible for the antiinflammatory action of the chamomile infusion observed here

Inhibition of Neutrophil Elastase and Metalloprotease-9 of Human Adenocarcinoma Gastric Cells by Chamomile (Matricaria recutita L.) Infusion / M. Bulgari, E. Sangiovanni, E. Colombo, O. Maschi, D. Caruso, E. Bosisio, M. Dell'Agli. - In: PHYTOTHERAPY RESEARCH. - ISSN 0951-418X. - 26:12(2012), pp. 1817-1822. [10.1002/ptr.4657]

Inhibition of Neutrophil Elastase and Metalloprotease-9 of Human Adenocarcinoma Gastric Cells by Chamomile (Matricaria recutita L.) Infusion

E. Sangiovanni;E. Colombo;O. Maschi;D. Caruso;E. Bosisio;M. Dell'Agli
2012

Abstract

This study investigated whether the antiinflammatory effect of chamomile infusion at gastric level could be ascribed to the inhibition of metalloproteinase-9 and elastase. The infusions from capitula and sifted flowers (250–1500mg/mL) and individual flavonoids (10mM) were tested on phorbol 12-myristate 13-acetate-stimulated AGS cells and human neutrophil elastase. The results indicate that the antiinflammatory activity associated with chamomile infusions from both the capitula and sifted flowers is most likely due to the inhibition of neutrophil elastase and gastric metalloproteinase-9 activity and secretion; the inhibition occurring in a concentration dependent manner. The promoter activity was inhibited as well and the decrease of metalloproteinase-9 expression was found to be associated with the inhibition of NF-kB driven transcription. The results further indicate that the flavonoid-7-glycosides, major constituents of chamomile flowers, may be responsible for the antiinflammatory action of the chamomile infusion observed here
Chamomile ; Matricaria recutita ; metalloprotease ; elastase ; gastritis ; flavonoids
Settore BIO/15 - Biologia Farmaceutica
Settore BIO/10 - Biochimica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/211202
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 15
social impact