The influence of bovine lymphocyte antigen (BoLA) complex polymorphism on subclinical progression of bovine leukaemia virus (BLV) infection was investigated in 41 Holstein-Friesian cows from two herds in Italy. All cows were seropositive for BLV and 22 had persistent lymphocytosis (PL). BoLA-A specificities were defined by serology, and class II haplotypes were defined based on restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR)-RFLP analysis of DQ and DR genes. Chi-square analysis revealed a significant and absolute association of haplotype DQA*3A-DQB*3A-DRB2*2A-DRB3.2*11 with resistance to PL (P chi 2 = 0.028, relative risk (RR) = 0.061). Consistent with this observation, multiple regression analysis revealed that animals carrying this haplotype had lower lymphocyte counts (P = 0.0057). By contrast, haplotype DQA*12-DQB*12-DRB2*3A-DRB3.2*8 was associated with susceptibility to PL (P chi 2 = 0.043, RR = 9.625) and increased lymphocyte counts (P = 0.0537). These results confirm the association of haplotype DQA*3A-DQB*3A-DRB2*2A-DRB3.2*11 with resistance to PL, and substantiate earlier findings of haplotype DQA*12-DQB*12-DRB2*3A-DRB3.2*8 as a risk factor for subclinical progression to PL in BLV-infected Holstein-Friesian cattle.
Association of BoLA class II haplotypes with subclinical progression of bovine leukaemia virus infection in Holstein-Friesian cattle / M. Zanotti, G. Poli, W. Ponti, M. Polli, M. Rocchi, E. Bolzani, M. Longeri, S. Russo, H.A. Lewin, M.J.T. Van Eijk. - In: ANIMAL GENETICS. - ISSN 0268-9146. - 27:5(1996 Oct), pp. 337-341.
|Titolo:||Association of BoLA class II haplotypes with subclinical progression of bovine leukaemia virus infection in Holstein-Friesian cattle|
ZANOTTI, MARTA (Primo)
POLI, GIORGIO (Secondo)
|Parole Chiave:||Bovine leukaemia virus; Bovine lymphocyte antigen; Disease association; Persistent lymphocytosis; Polymerase chain reaction-restriction fragment length polymorphism|
|Settore Scientifico Disciplinare:||Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico|
Settore VET/05 - Malattie Infettive degli Animali Domestici
|Data di pubblicazione:||ott-1996|
|Appare nelle tipologie:||01 - Articolo su periodico|