In this work a novel three-dimensional ostechondral substitute is proposed that is made of an inorganic/organic hybrid material, namely collagen/hydroxyapatite. The two components of the substitute have been characterized separately. The inorganic part, a hydroxyapatite scaffold, was fabricated by a polymer sponge templating method using a reactive sub-micron powder synthesized in our laboratory by hydroxide precipitation sol-gel route. The organic part, a collagen scaffold, was fabricated by a freeze-dying technique varying design parameters. Both the parts were analysed by scanning electron microscopy and their mechanical properties assessed by compression tests. The hydroxyapatite scaffold showed a high and highly interconnected porosity and a mechanical strength equal to 0.55 MPa, higher than those reported in literature. The collagen scaffolds were seeded by chondrocytes, processed for histology analysis and tested in compression. The biological tests proved the ability of the scaffolds to be positively populated by chondrocytes and the mechanical analysis showed that the mechanical strength of the scaffolds significantly increased after 3 weeks of culture.

Development and mechanical characterization of a collagen/hydroxyapatite bilayered scaffold for ostechondral defect replacement / F. Gervaso, F. Scalera, S.K. Padmanabhan, A. Licciulli, D. Deponti, A. Di Giancamillo, C. Domeneghini, G.M. Peretti, A. Sannino (KEY ENGINEERING MATERIALS). - In: Bioceramics 23 / [a cura di] E.S. Kayali, G. Goller, I. Akin. - Istanbul : Trans Tech publications, 2012. - ISBN 9783037852552. - pp. 890-895 (( Intervento presentato al 23. convegno Symposium and annual meeting of International society for ceramics in medicine (ISCM) tenutosi a Istanbul nel 2011 [10.4028/www.scientific.net/KEM.493-494.890].

Development and mechanical characterization of a collagen/hydroxyapatite bilayered scaffold for ostechondral defect replacement

A. Di Giancamillo;C. Domeneghini;G.M. Peretti
Penultimo
;
2012

Abstract

In this work a novel three-dimensional ostechondral substitute is proposed that is made of an inorganic/organic hybrid material, namely collagen/hydroxyapatite. The two components of the substitute have been characterized separately. The inorganic part, a hydroxyapatite scaffold, was fabricated by a polymer sponge templating method using a reactive sub-micron powder synthesized in our laboratory by hydroxide precipitation sol-gel route. The organic part, a collagen scaffold, was fabricated by a freeze-dying technique varying design parameters. Both the parts were analysed by scanning electron microscopy and their mechanical properties assessed by compression tests. The hydroxyapatite scaffold showed a high and highly interconnected porosity and a mechanical strength equal to 0.55 MPa, higher than those reported in literature. The collagen scaffolds were seeded by chondrocytes, processed for histology analysis and tested in compression. The biological tests proved the ability of the scaffolds to be positively populated by chondrocytes and the mechanical analysis showed that the mechanical strength of the scaffolds significantly increased after 3 weeks of culture.
biological test; bone; cartilage; compression test; tissue engineering
Settore MED/33 - Malattie Apparato Locomotore
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/209580
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