A cell line derived from human embryonic epithelium (EUE cells) shows an enhanced expression of a 33 kDa protein when adapted to grow in a hypertonic medium containing 0.246 M NaCl (1.8 x the isotonic concentration). The maximum amount of this protein, followed by SDS-PAGE electrophoresis, was found after 4 days of adaptation; thereafter, the protein band remained fairly constant up to 30 days. When the cells were transferred back to a medium containing 0.137 m NaCl (isotonic medium), the protein pattern reverted to that of control cells. This protein is mainly localized in the cytosol, although a small part is associated with the 150 000 g pellet and needs detergents to be extracted. The molecular weight and the cellular location suggest a possible analogy with the so-called amphitropic proteins, that are known to interact with both the epidermal growth factor receptor and hydrophobic structures, such as the membrane phospholipids and the cytoskeletal components.
A 33 KDa Protein Band is enhanced during long term adaptation of EUE cells to a hypertonic medium / A.E.M. Giuliani, A. Ferraretto, A.M. Fuhrman Conti, L. De grada, A. Fraschini, C. Pellicciari, M.G. Manfredi Romanini. - In: CELL BIOCHEMISTRY AND FUNCTION. - ISSN 0263-6484. - 9:2(1991 Apr), pp. 87-94.
|Titolo:||A 33 KDa Protein Band is enhanced during long term adaptation of EUE cells to a hypertonic medium|
GIULIANI, ATTILIA ENRICA MARIA (Primo)
FERRARETTO, ANITA (Secondo)
|Parole Chiave:||HYPERTONIC STRESS ; EUE CELLS ; SDS-PAGE|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||apr-1991|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/cbf.290090204|
|Appare nelle tipologie:||01 - Articolo su periodico|