A cell line derived from human embryonic epithelium (EUE cells) shows an enhanced expression of a 33 kDa protein when adapted to grow in a hypertonic medium containing 0.246 M NaCl (1.8 x the isotonic concentration). The maximum amount of this protein, followed by SDS-PAGE electrophoresis, was found after 4 days of adaptation; thereafter, the protein band remained fairly constant up to 30 days. When the cells were transferred back to a medium containing 0.137 m NaCl (isotonic medium), the protein pattern reverted to that of control cells. This protein is mainly localized in the cytosol, although a small part is associated with the 150 000 g pellet and needs detergents to be extracted. The molecular weight and the cellular location suggest a possible analogy with the so-called amphitropic proteins, that are known to interact with both the epidermal growth factor receptor and hydrophobic structures, such as the membrane phospholipids and the cytoskeletal components.

A 33 KDa Protein Band is enhanced during long term adaptation of EUE cells to a hypertonic medium / A.E.M. Giuliani, A. Ferraretto, A.M. Fuhrman Conti, L. De grada, A. Fraschini, C. Pellicciari, M.G. Manfredi Romanini. - In: CELL BIOCHEMISTRY AND FUNCTION. - ISSN 0263-6484. - 9:2(1991 Apr), pp. 87-94.

A 33 KDa Protein Band is enhanced during long term adaptation of EUE cells to a hypertonic medium

A.E.M. Giuliani
Primo
;
A. Ferraretto
Secondo
;
1991

Abstract

A cell line derived from human embryonic epithelium (EUE cells) shows an enhanced expression of a 33 kDa protein when adapted to grow in a hypertonic medium containing 0.246 M NaCl (1.8 x the isotonic concentration). The maximum amount of this protein, followed by SDS-PAGE electrophoresis, was found after 4 days of adaptation; thereafter, the protein band remained fairly constant up to 30 days. When the cells were transferred back to a medium containing 0.137 m NaCl (isotonic medium), the protein pattern reverted to that of control cells. This protein is mainly localized in the cytosol, although a small part is associated with the 150 000 g pellet and needs detergents to be extracted. The molecular weight and the cellular location suggest a possible analogy with the so-called amphitropic proteins, that are known to interact with both the epidermal growth factor receptor and hydrophobic structures, such as the membrane phospholipids and the cytoskeletal components.
HYPERTONIC STRESS ; EUE CELLS ; SDS-PAGE
Settore BIO/10 - Biochimica
apr-1991
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/207448
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