We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygosity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possible preferential involvement of some tumor suppressor genes in endometrial carcinogenesis. The analysis was performed on tumoral tissue and on a corresponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37. 50%), 11q13-q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21. 3 (37.04%). We defined a 1-cM minimal common deletion in 11q13-q14 between D11S911 and D11S937 markers. A statistical analysis revealed a positive correlation between LOH of 11q13-q14 and clinicopathological data
Losses of heterozygosity in endometrial adenocarcinomas : positive correlations with histopathological parameters / S.M. Sirchia, E. Sironi, F.R. Grati, P. Serafini, I. Garagiola, F. Rossella, F. Dulcetti, G. Pardi, S. Garsia, G. Simoni. - In: CANCER GENETICS AND CYTOGENETICS. - ISSN 0165-4608. - 121:2(2000 Sep), pp. 156-162. [10.1016/S0165-4608(00)00240-5]
Losses of heterozygosity in endometrial adenocarcinomas : positive correlations with histopathological parameters
S.M. SirchiaPrimo
;F.R. Grati;I. Garagiola;F. Rossella;G. Pardi;G. SimoniUltimo
2000
Abstract
We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygosity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possible preferential involvement of some tumor suppressor genes in endometrial carcinogenesis. The analysis was performed on tumoral tissue and on a corresponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37. 50%), 11q13-q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21. 3 (37.04%). We defined a 1-cM minimal common deletion in 11q13-q14 between D11S911 and D11S937 markers. A statistical analysis revealed a positive correlation between LOH of 11q13-q14 and clinicopathological dataFile | Dimensione | Formato | |
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