The identification of hepatitis C virus (HCV) in semen remains controversial and that of hepatitis G virus (HGV) or GB virus C (GBV-C) has never been investigated. Serum and semen from 90 anti-HCV-positive drug users were tested (27 infected with HIV) for HCV and HGV/GBV-C RNAs by polymerase chain reaction (PCR) assay, hybridization, and sequence analysis. Semen was processed into round cells, seminal plasma, and spermatozoa. Fifty-six patients were HCV-viremic, but HCV-RNA was not identified in their seminal fractions. However, PCR inhibitors were found in the semen of 34 of these men. Twenty-eight patients had HGV/GBV-C RNA in their blood and for 24 of them, ejaculates were available for analysis. HGV/GBV-C RNA was found in the seminal plasma of 6 of 12 samples free from PCR inhibitors. These results agree with the low risk of sexual transfer of HCV and provide preliminary evidence for the presence of HGV/GBV-C in semen.
|Titolo:||Absence of hepatitis C virus and detection of hepatitis G virus/GB virus C RNA sequences in the semen of infected men|
SEMPRINI, AUGUSTO ENRICO (Primo)
|Parole Chiave:||Hepacivirus; Viral Nonstructural Proteins; Humans; Hepatitis, Viral, Human; Nucleic Acid Hybridization; RNA, Viral; HIV-1; Flaviviridae; Polymerase Chain Reaction; DNA, Complementary; Base Sequence; Sequence Alignment; AIDS-Related Opportunistic Infections; Adult; DNA Primers; Molecular Sequence Data; Sequence Analysis, RNA; Semen; Hepatitis C; Male; Substance Abuse, Intravenous|
|Settore Scientifico Disciplinare:||Settore MED/40 - Ginecologia e Ostetricia|
|Data di pubblicazione:||apr-1998|
|Digital Object Identifier (DOI):||10.1086/515257|
|Appare nelle tipologie:||01 - Articolo su periodico|