Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). However, the mechanisms are incompletely understood. We show here that HIV-1 suppression is mediated by soluble factors released by MDM stimulated with physiologically significant concentrations of LPS. LPS-conditioned supernatants from MDM inhibited HIV-1 replication in both MDM and T cells. Depletion of C-C chemokines (RANTES, MIP-1 alpha, and MIP-1 beta) neutralized the ability of LPS-conditioned supernatants to inhibit HIV-1 replication in MDM. A combination of recombinant C-C chemokines blocked HIV-1 infection as effectively as LPS. Here, we report an inhibitory effect of C-C chemokines on HIV replication in primary macrophages. Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes.
|Titolo:||C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells|
|Parole Chiave:||Virus Replication; Macrophages; Tumor Necrosis Factor-alpha; Chemokines; Humans; Receptors, CCR5; Receptors, HIV; HIV-1; Antigens, CD14; Polymerase Chain Reaction; DNA, Viral; Receptors, Cytokine; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Interleukin-6; Lipopolysaccharides; Macrophage Inflammatory Proteins; Up-Regulation; Chemokine CCL4; T-Lymphocytes; Chemokine CCL5|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||3-mar-1997|
|Digital Object Identifier (DOI):||10.1084/jem.185.5.805|
|Appare nelle tipologie:||01 - Articolo su periodico|