The genetic variation of bovine viral diarrhoea virus (BVDV) was studied by comparative nucleotide sequence analysis of 26 Italian field strains collected during the period 1995-2000 in 18 cattle herds. A fragment within the 5′-untranslated region (UTR) was sequenced directly from gel-purified products obtained by reverse transcription polymerase chain reaction. BVDV-1b (n=14), -1c (n=1), -1d (n=1) and BVDV-2 (n=2) strains have been isolated. Most herds were infected by BVDV-1b. Pairwise similarity and cluster analysis of the remaining BVDV-1 isolates (n=8) did not provide a clear-cut assignation to defined BVDV-1 groups. This is the first time that a BVDV-2 isolation was reported in Italy. Among BVDV-2 reference strains, Italian BVDV-2 isolates showed the highest sequence similarity with the CD87 strain. Both BVDV-2 strains were isolated in two healthy animals from different herds. The 5′-UTR sequence of one of the two BVDV-2 strains was identical to a German BVDV field strain. Complete nucleotide homology was found only among BVDV strains isolated from the same herd, showing a herd-specific clustering. Moreover, 99.6% homology was observed between strains from herds linked by livestock trade. Despite the small number of BVDV isolates analysed, it revealed a high level of genetic diversity among Italian field BVDV strains.
Distribution pattern of bovine viral diarrhoea virus strains in intensive cattle herds in Italy / C. Luzzago, C. Bandi, V. Bronzo, G. Ruffo, A. Zecconi. - In: VETERINARY MICROBIOLOGY. - ISSN 0378-1135. - 83:3(2001 Nov 26), pp. 265-274.
|Titolo:||Distribution pattern of bovine viral diarrhoea virus strains in intensive cattle herds in Italy|
LUZZAGO, CAMILLA (Primo)
BANDI, CLAUDIO (Secondo)
RUFFO, GIANFRANCO (Penultimo)
ZECCONI, ALFONSO (Ultimo)
|Parole Chiave:||Bovine viral diarrhoea virus; Cattle-viruses; Epidemiology; Genetic typing; PCR|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Data di pubblicazione:||26-nov-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0378-1135(01)00429-1|
|Appare nelle tipologie:||01 - Articolo su periodico|