Maternal contamination of fetal DNA represents a major problem when highly sensitive molecular techniques are used in the prenatal diagnosis of genetic diseases. For this reason, we have studied the possibility of using DNA isolated from syncytiotrophoblast vesicles as a target of gene amplification (PCR). Three PCR systems were selected which included a repetitive 149 bp fragment of the Y chromosome, the VNTR locus D1S80, and a portion of the beta-globin gene. The results of these experiments indicate that DNA isolated from syncytiotrophoblast vesicles is free of maternal contamination and is suitable for gene amplification and DNA analysis.
Applicability of DNA isolated from syncytiotrophoblast vesicles to gene amplification and molecular analysis / G. Colucci, E. Pesenti, E. Molteni, A. Lobbiani, C. De Andreis, S. Pariani, F. Rossella, A. E. Semprini, G. Simoni. - In: PRENATAL DIAGNOSIS. - ISSN 0197-3851. - 13:5(1993 May), pp. 335-340. [10.1002/pd.1970130504]
Applicability of DNA isolated from syncytiotrophoblast vesicles to gene amplification and molecular analysis
A.E. Semprini;
1993
Abstract
Maternal contamination of fetal DNA represents a major problem when highly sensitive molecular techniques are used in the prenatal diagnosis of genetic diseases. For this reason, we have studied the possibility of using DNA isolated from syncytiotrophoblast vesicles as a target of gene amplification (PCR). Three PCR systems were selected which included a repetitive 149 bp fragment of the Y chromosome, the VNTR locus D1S80, and a portion of the beta-globin gene. The results of these experiments indicate that DNA isolated from syncytiotrophoblast vesicles is free of maternal contamination and is suitable for gene amplification and DNA analysis.
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