PTX3 is a prototypic long pentraxin expressed by various cell types, most prominently monocytes and endothelial cells, in response to interleukin-1 (IL-1), tumor necrosis factor (TNF) and bacterial products. In the present report, we show that interferon- (IFN-) inhibits the expression of the PTX3 gene induced by exposure to IL-1, TNF or lipopolysaccharide in human monocytes. This effect is dose dependent and observable when IFN- is added from 24 h before up to 3 h after the addition of IL-1. While the time course of the IL-1-induced PTX3 mRNA expression is not affected, IFN- reduces the stability of the PTX3 mRNA as well as its transcription. The inhibition of PTX3 expression is restricted to monocytes in that no inhibition occurs in cytokine-stimulated fibroblasts and endothelial cells. Under the same conditions, as expected, IFN- augmented monocyte chemotactic protein-1 expression in the same cell preparations. PTX3 protein secretion by activated monocytes is also suppressed by exposure to IFN-. Altogether, these data identify a negative pathway of regulation mediated by IFN-, which may occur under inflammatory conditions.
Interferon-gamma inhibits expression of the long pentraxin PTX3 in human monocytes / N. Polentarutti, G. Picardi, A. Basile, S. Cenzuales, A. Rivolta, C. Matteucci, G. Peri, A. Mantovani, M. Introna. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 28:2(1998 Feb), pp. 496-501.
|Titolo:||Interferon-gamma inhibits expression of the long pentraxin PTX3 in human monocytes|
MANTOVANI, ALBERTO (Penultimo)
|Parole Chiave:||Acute-phase reactants; Gene regulation; Human; Inflammation; Monocyte|
|Settore Scientifico Disciplinare:||Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio|
|Data di pubblicazione:||feb-1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/(SICI)1521-4141(199802)28:02<496::AID-IMMU496>3.0.CO;2-V|
|Appare nelle tipologie:||01 - Articolo su periodico|